Mouse IGFBP3 ELISA Kit (ab100692)
Key features and details
- Sensitivity: 70 pg/ml
- Range: 54.87 pg/ml - 40000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
-
Product name
Mouse IGFBP3 ELISA Kit
See all IGFBP3 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 70 pg/ml -
Range
54.87 pg/ml - 40000 pg/ml -
Recovery
89 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 105.5 93% - 112% Serum 85.83 73% - 94% Plasma 76.81 71% - 84% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s IGFBP3 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IGFBP3 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IGFBP3 coated on a 96- well plate. Standards and samples are pipetted into the wells and IGFBP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGFBP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
Platform
Microplate
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Mouse IGFBP3 2 vials IGFBP3 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IGFBP3 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Relevance
Insulin like growth factor binding protein 3 (IGFBP3) is a member of the superfamily of insulin like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGFBP) and at least four low affinity binding proteins referred to as IGFBP related proteins (IGFBPrP). The IGFBP members are cysteine rich proteins with conserved cysteine residues clustered in the amino terminal and the carboxy terminal regions of the molecule. The cDNA sequence encoding the mature human IGFBP3 is fused to the signal peptide of CD33. Human IGFBP3 is the major IGF binding protein in plasma where it exists in a ternary complex with IGFI or IGFII and an acid labile subunit. IGFBPs hold a central position in IGF ligand-receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment. Insulin like growth factor binding protein 3 (IGFBP3) can modulate the mitogenic and metabolic effects of the insulin like growth factors (IGFs). Insulin like growth factor binding protein 3 (IGFBP3) is expressed in multiple tissues. The highest expression level is found in the non paranchymal cells of the liver. The expression levels are higher during extrauterine life and peak during puberty. Insulin like growth factors (IGFs) and IGF binding proteins (IGFBPs) play important roles in cell growth and differentiation. IGFBP3 is one of the factors in serum that is responsible for high serum induced apoptosis in PC3 cells, a prostate cancer cell line. IGFBP3 is important in controlling glucose homeostasis with increased urinary levels in type I diabetes with persistent microalbuminuria. -
Cellular localization
Secreted -
Alternative names
- Acid stable subunit of the 140 K IGF complex
- Binding protein 29
- Binding protein 53
see all -
Database links
- Entrez Gene: 16009 Mouse
- SwissProt: P47878 Mouse
Images
-
IGFBP3 in mouse tissue lysates extracted with RIPA buffer. Data from kidney lysates diluted 1/50-1/250 (protein concentration 0.25-0.05 mg x mL-1) and lung diluted 1/10 (0.75 mg x mL-1) (duplicates +/- SD).
-
IGFBP3 in mouse biological fluids (duplicates +/- SD). Plasma and serum tested in the dilution range of 1/50-1/250, urine tested undiluted, rat and human samples (serum and plasma) were below level of detection at 1/10 dilution.
-
Representative Standard Curve using ab100692
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab100692 has been referenced in 3 publications.
- Miyagawa K et al. Osteoclast-derived IGF1 is required for pagetic lesion formation in vivo. JCI Insight 5:N/A (2020). PubMed: 32078587
- Suman S et al. Space radiation exposure persistently increased leptin and IGF1 in serum and activated leptin-IGF1 signaling axis in mouse intestine. Sci Rep 6:31853 (2016). PubMed: 27558773
- Suman S et al. Protracted upregulation of leptin and IGF1 is associated with activation of PI3K/Akt and JAK2 pathway in mouse intestine after ionizing radiation exposure. Int J Biol Sci 11:274-83 (2015). PubMed: 25678846