Overview

  • Product name
    Mouse IGFBP6 ELISA Kit
    See all IGFBP6 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 6 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    91 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 93.12 83% - 103%
    Serum 89.43 81% - 99%
    Plasma 90.76 82% - 101%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IGFBP6 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IGFBP6 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for mouse IGFBP6 coated on a 96- well plate. Standards and samples are pipetted into the wells and IGFBP6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGFBP6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get better reproducibility in only 90 minutes with Mouse IGFBP6 ELISA Kit (ab206305) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    80x HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Mouse IGFBP6 2 vials
    IGFBP6 Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse IGFBP6 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • Sequence similarities
    Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • Post-translational
    modifications
    O-linked glycans consist of hexose (probably Gal), N-acetylhexosamine (probably GalNAc) and sialic acid residues. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylated on one site only in the region AA 143-168 in cerebrospinal fluid.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • IBP 6
    • IBP-6
    • IBP6
    • IBP6_HUMAN
    • IGF binding protein 6
    • IGF-binding protein 6
    • IGFBP 6
    • IGFBP-6
    • IGFBP6
    • Insulin like growth factor binding protein 6
    • Insulin-like growth factor-binding protein 6
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab100694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab100694

  • Representative Standard Curve using ab100694

Protocols

References

ab100694 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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