|Sample type||Average %||Range|
|Cell culture supernatant||93.12||83% - 103%|
|Serum||89.43||81% - 99%|
|Plasma||90.76||82% - 101%|
Abcam’s IGFBP6 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IGFBP6 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IGFBP6 coated on a 96- well plate. Standards and samples are pipetted into the wells and IGFBP6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGFBP6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get better reproducibility in only 90 minutes with Mouse IGFBP6 ELISA Kit (ab206305) from our SimpleStep ELISA® range.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|80x HRP-Streptavidin Concentrate||1 x 200µl|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Mouse IGFBP6||2 vials|
|IGFBP6 Microplate (12 x 8 wells)||1 unit|
|Recombinant Mouse IGFBP6 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100694 has not yet been referenced specifically in any publications.