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Read our guarantee »Overview
Product name
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Chromeo™ 642)
See all IgG products (1703) ...
Target species
Mouse
Specificity
ab60318 reacts with whole molecular mouse IgG and the light chains of other mouse immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins observed.
Tested applications
ICC/IFmore details
Immunogen
Mouse IgG.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C. Store in the dark. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 2mM Sodium Azide
Constituents: 0.15M Sodium chloride, 0.01M Potassium phosphate, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Conjugation notes
Chromeo™ 642 is spectrally similar to Cy5®. Conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching, and have absorption and emission maxima of approximately 642 and 666 nm which are compatible with common excitation sources and filter sets. Molar extinction Coefficient: 180,000 M-1cm-1 (measured at Amax) Quantum Yield: ~ 7 % Excitation Wavelength Range: 630 to 655 nm Emission Wavelength Range: 650 to 670 nm
Clonality
Polyclonal
Isotype
IgG
Research areas
Secondary antibodies >> anti-Mouse >> IgG >> Fluorophore >> Chromeo™ 642
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Chromeo™ 642) (ab60318)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab60318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
ICC/IF
Application notes
ICC/IF: 1/1000 - 1/2000.
Plate-based assays: 1/800 to 1/1000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Chromeo™ 642) images:
Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Chromeo™ 642) (ab60318)
ab60318 staining a mouse monoclonal antibody to alpha Tubulin in HeLa cells by Immunocytochemistry/Immunofluorescense with a DAPI nuclear counterstain.
References for Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Chromeo™ 642) (ab60318)
ab60318 has not yet been referenced specifically in any publications.
Publishing research using ab60318? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"