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Product Name 

Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (AP)

Product type 

Secondary antibodies

Description 

Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (AP)

Immunogen 

mouse IgG whole molecule

Tested applications 
(see key)

Dot, ELISA, IHC-Fr, IHC-P, IM, WB

Application notes 
(see key)

Recommended dilutions
suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency

Please refer to the relevant conjugation for dilutions specific for this product:

Conjugate

ELISA

Blotting

Flow Cytometry

IHC

Fluorescein

nd

nd

50 - 200

200 - 1,000

Rhodamine, Texas RedTM

nd

nd

50 - 200

200 - 1,000

R-Phycoerythrin

nd

nd

100 - 200

nd

Cyanines

nd

nd

400

100 - 1,000

Biotin

5,000 - 20,000

3,000 - 10,000

nd

100 - 1,000

Peroxidase

4,000 - 10,000

2,000 - 5,000

nd

200 - 2,000

Alkaline Phosphatase

1,000 - 2,000

500 - 1,000

nd

100 - 1,000

Research areas 

Secondary antibodies >> anti-Mouse >> IgG >> Enzyme >> Alkaline Phos
Immunology >> Immunoglobulins >> Heavy Chain >> IgG

 

Alternative names Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab6821)

Raised in 

Donkey

Clonality 

Polyclonal

Purity 

IgG fraction

Conjugation notes 

Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

Storage buffer 

0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA

Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS

Purification notes 

This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads.

Form 

Liquid

Conjugation 

Alkaline Phosphatase

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

 

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