Recombinant

Anti-Mouse IgG1 antibody [RM106] (ab190481)

Overview

  • Product name
    Anti-Mouse IgG1 antibody [RM106]
  • Description
    Rabbit monoclonal [RM106] to Mouse IgG1
  • Tested applications
    Suitable for: ICC, WB, ELISA, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Rat, Human
  • Immunogen

    Full length protein corresponding to Mouse IgG1.

  • Positive control
    • Mouse IgG1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 48% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    RM106
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 0.5 - 2 µg/ml.
WB Use a concentration of 0.1 - 0.5 µg/ml.
ELISA Use a concentration of 0.0005 - 0.2 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.

Target

  • Relevance
    Immunoglobulin G (IgG), is one of the most abundant protein in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B-lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • Ig gamma-1 chain C region secreted form antibody
    • Ig gamma-1 chain C region, membrane-bound form antibody
    • IgG1 antibody
    • Igh-4 antibody
    • Ighg1 antibody
    • immunoglobulin heavy constant gamma 1 (G1m marker) [ Mus musculus (house mouse) ] antibody
    • immunoglobulin heavy constant gamma 1 (G1m marker) antibody
    • VH7183 antibody
    see all

Images

  • All lanes : Anti-Mouse IgG1 antibody [RM106] (ab190481) at 0.2 µg/ml

    Lane 1 : Mouse IgG1, non-reduced
    Lane 2 : Mouse IgG1, reduced

    Lysates/proteins at 0.02 µg per lane.

    ab190481 reacted to non-reduced IgG1 (~150 kDa) stronger thanthe reduced γ1(~50 kDa).

References

ab190481 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab190481.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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