Mouse IgG1 [CT6] - Isotype control (ab81216)

Overview

  • Product name
    Mouse IgG1 [CT6] - Isotype control
    See all Mouse isotype controls
  • Specificity
    The antibody reacts with a Guinea pig lymphocyte subset probably analog to human CD8 (cytotoxic/suppressor) subset. Although this monoclonal originally was developed for the detection of a Guinea pig lymphocyte subset, it also can be used as a negative control for the mouse IgG1 subclass. It is sufficient for 100 tests. The antibody does not react with the 170-180 kD glycoprotein which is strongly correlated with the degree of Multi Drug Resistance. The antibody is sufficient for 100 tests.
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Guinea pig
  • Immunogen

    The details of the immunogen for this antibody are not available.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    CT6
  • Isotype
    IgG1
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab81216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IHC-P 1/20.

References for Mouse IgG1 [CT6] - Isotype control (ab81216)

This product has been referenced in:
  • Schehr JL  et al. High Specificity in Circulating Tumor Cell Identification Is Required for Accurate Evaluation of Programmed Death-Ligand 1. PLoS One 11:e0159397 (2016). Read more (PubMed: 27459545) »
  • Scheper RJ  et al. Monoclonal antibody JSB-1 detects a highly conserved epitope on the P-glycoprotein associated with multi-drug-resistance. Int J Cancer 42:389-94 (1988). Read more (PubMed: 2458323) »

See all 3 Publications for this product

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We have not heard other reports of this, but it is possible the azide in the antibody, which is present at 0.1% in the stock solution, does have an effect on the tumor growth. Confirming this would require an exp...

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I have attached the certificate of compliance for ab290 and ab81216, with the lot numbers specified.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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