Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Overview

  • Product nameMouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control
  • SpecificityThis Mouse IgG1 Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments.
  • Tested applicationsSuitable for: Flow Cyt, ICC, IHC-Pmore details
  • General notes

    Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation.

     

    The control performed appropriately in all materials and platforms that were tested.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituent: 99% HBS
  • Concentration information loading...
  • PurityAmmonium Sulphate Precipitation
  • ClonalityMonoclonal
  • Clone number15-6E10A7
  • IsotypeIgG1
  • Light chain typekappa

    Applications

    Our Abpromise guarantee covers the use of ab170190 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use a concentration of 1 µg/ml.
    ICC Use a concentration of 1 µg/ml.
    IHC-P Use at an assay dependent concentration. PubMed: 26627833

    Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control images

    • Flow cytometry experiments with 4% PFA fixed HeLa (Human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast) were performed with the IgG1 Isotype Control Antibody (red) and no primary antibody negative control (black).

      An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.

    • Immunocytochemistry (ICC) experiments with NIH-3T3 (mouse sarcoma), COS7 (monkey kidney fibroblast), H9C2 (rat myoblast) and Hela (human adenocarcinoma) were performed with the IgG1 Isotype Control Antibody (top), no primary antibody negative control (middle), and ab14705 as a positive control (bottom).

      An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.

    • Fluorescence ELISA.

      An isotyping ELISA was performed by coating a 96-well plate with 1.25 ug/mL of the IgG1 Isotype Control Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG. This experiment verifies that the primary antibody's isotype is correct and that it is successfully bound by the secondary antibody.

    References for Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    This product has been referenced in:
    • Cui Y  et al. The miR-20-Rest-Wnt signaling axis regulates neural progenitor cell differentiation. Sci Rep 6:23300 (2016). Flow Cyt . Read more (PubMed: 26996236) »
    • Landa-Solís C  et al. Cryopreserved CD90+ cells obtained from mobilized peripheral blood in sheep: a new source of mesenchymal stem cells for preclinical applications. Cell Tissue Bank N/A:N/A (2015). Read more (PubMed: 26220398) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"