Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (Alexa Fluor® 594) (ab178000)

Overview

  • Product name
    Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (Alexa Fluor® 594)
    See all IgG isotype controls
  • Conjugation
    Alexa Fluor® 594. Ex: 590nm, Em: 617nm
  • Tested applications
    Suitable for: ICC/IFmore details
  • General notes

    Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation. The Control performed appropriately in all materials and platforms that were tested. 

    Product was previously marketed under the MitoSciences sub-brand.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: 68% PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Monoclonal
  • Clone number
    15-6E10A7
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas
  • Cellular localization
    Secreted
  • Alternative names
    • Ig gamma 1 chain C region
    • Ig gamma 2 chain C region
    • Ig gamma 3 chain C region
    • Ig gamma 4 chain C region
    • IgG
    • IGHG1
    • IGHG2
    • IGHG3
    • IGHG4
    • Immunoglobin heavy constant gamma 1
    • Immunoglobulin G
    see all

Applications

Our Abpromise guarantee covers the use of ab178000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.

Images

  • Immunocytochemistry data for ab178000. All panels show HeLa cells stained with DAPI as well as; a no primary control (A), ab178000 at 0.1 μg/mL (B), an Alexa 594 anti-SDHA conjugated antibody (ab170172) at 0.1 μg/mL (C).

    Cells were PFA fixed and stored in 0.02%azide/PBS in 4°C, then blocked with 10%GS for 2 hours, primary was diluted in 10%GS and incubated for 2 hours, cells were washed 3x with 1%GS and DAPI was added for 10 minutes in 1%GS.

References

ab178000 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Questions for ab178000.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up