Mouse IgG1, kappa monoclonal [MG1-45] - BSA/Azide free (ab18447)

Overview

  • Product name
    Mouse IgG1, kappa monoclonal [MG1-45] - BSA/Azide free
    See all Mouse isotype controls
  • Tested applications
    Suitable for: ELISA, IHC-P, IHC-Fr, Functional Studies, Flow Cyt, WB, IPmore details
  • Immunogen

    Chemical/ Small Molecule (Trinitrophenol) conjugated to KLH.

  • General notes

    The ab18447, MG1-45 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab18447 is 'LEAF Purified'. This is a low endotoxin azide free formulation containing no preservative. The antibody is 0.2 µm filter sterilized and the endotoxin level is <0.01 ng/µg protein.
  • Isotype control notes
    The ab18447, MG1-45 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
  • Clonality
    Monoclonal
  • Clone number
    MG1-45
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. PubMed: 18006877
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. Use at an assay dependent dilution for surface or intracellular staining.
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

References for Mouse IgG1, kappa monoclonal [MG1-45] - BSA/Azide free (ab18447)

This product has been referenced in:
  • Levet S  et al. An ancestral retroviral protein identified as a therapeutic target in type-1 diabetes. JCI Insight 2:N/A (2017). IHC-P . Read more (PubMed: 28878130) »
  • Hanamura T  et al. Human 3ß-hydroxysteroid dehydrogenase type 1 in human breast cancer: clinical significance and prognostic associations. Cancer Med 5:1405-15 (2016). IHC . Read more (PubMed: 27139182) »

See all 7 Publications for this product

Product Wall

I can confirm that ab18447 Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control would be a suitable isotype control for use with ab46678 CD14 antibody [MEM-18]. It has been tested and therefore covered by the guarantee in functional studies.
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Tom is currently away from the office. Thus, I will be helping you. My name is Sybille.

As for ab18447, it is a protein G purified antibody that comes in PBS without any preservatives. As per datasheet, ab18447 is 'LEAF Purified'. This is a lo...

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I am very pleased to hear you would like to accept our offer and test these products in flow cytometry. Each code will give you the value listed off your next order before the expiration date. To redeem this offer, please submit an Abreview for flow cy...

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The concentration of this lot is GR91861-1 is 0.85mg/ml. An appropriate isotype control would be ab91353, Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control. This product is shipped with a concentration of 1mg/ml. http://www.abcam.com/Mouse-IgG...

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Thank you for contacting us.

We do not currently have a pan- mouse IgG isotype control suitable for in vivo use on mouse. We do have isotype controls for each IgG subtype which have beenLEAF Purified. This is a low endotoxin azide free for...

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Thank youfor your inquiry.

I can confirm that we do have three anti NS1 antibodies for Dengue virus in our catalog..

Isotype control are not included and have to be purchased separately.

ab41616 (Anti-Dengue Virus NS1 glyc...

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Here are the MSDS forms you requested. Please contact us again if you need anything further.

Thank you for your inquiry and I apologize for the delay in this reply. Yes, ab18447 is a suitable isotype control for ab17199. http://www.abcam.com/Mouse-IgG1-kappa-monoclonal-MG1-45-Isotype-control-ab18447.html I hope this inform...

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Thank you for contacting us. Could you please specify which product you would be interested in buying? I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us. I can confirm that product ab91353 can also be used as normal isotype control with human cells or in applications as stated on the datasheet. I have also sent an email to my colleague in lab about your requir...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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