Mouse IgG1, kappa monoclonal [MOPC-21] - Isotype Control (Low endotoxin, Azide Free) (ab18437)

Overview

  • Product name
    Mouse IgG1, kappa monoclonal [MOPC-21] - Isotype Control (Low endotoxin, Azide Free)
    See all Mouse isotype controls
  • Tested applications
    Suitable for: Flow Cyt, WB, IP, IHC-P, IHC-Fr, ICC/IFmore details
  • General notes
    The ab18437, MOPC-21 immunoglobulin has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues.


    The ab18437, MOPC-21 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemical staining, bioassay, and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
    0.2µm filter sterilized. Endotoxin level is <0.01 ng/µg protein.
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Ab18437 is low endotoxin and azide free. The antibody was purified by affinity chromatography.
  • Isotype control notes
    The ab18437, MOPC-21 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemical staining, bioassay, and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
  • Clonality
    Monoclonal
  • Clone number
    MOPC-21
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. Suitable for surface or intracellular staining.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

References

ab18437 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

The concentration is determined as follows:

When a new lot is produced the lab takes an aliquot and measure the OD of that aliquot multiple times and a mean is calculated. Then a second aliquot is measured independently, also several times a...

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Thank you for your enquiry. The antibody concentration is 1 mg/ml. Please do not hesitate to contact us if you have additional questions.

Thank you for contacting us for technical support, I'm sorry to hear you are experiencing problems with ab18437. I think the problem might be due to the fact that ab18437 is pure IgG1 kappa, while ab20034 is made of a mixture of kappa and lambda. Th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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