Recombinant

Anti-Mouse IgG2a antibody [RM107] (ab190463)

Overview

  • Product name
    Anti-Mouse IgG2a antibody [RM107]
    See all Mouse IgG2a primary antibodies
  • Description
    Rabbit monoclonal [RM107] to Mouse IgG2a
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IP, ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Full length protein corresponding to Mouse IgG2a.

  • Positive control
    • Mouse IgG2a kappa

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 48% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Protein A affinity purified from an animal origin-free and protein-free culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    RM107
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use a concentration of 0.5 - 2 µg/ml.
IP Use at an assay dependent concentration.
ELISA Use a concentration of 0.005 - 0.2 µg/ml.
WB Use a concentration of 0.1 - 0.5 µg/ml.

Non-reducing conditions only

IHC-P Use a concentration of 0.5 - 2 µg/ml.

Target

Images

  • All lanes : Anti-Mouse IgG2a antibody [RM107] (ab190463) at 0.2 µg/ml

    Lane 1 : Mouse IgG2a kappa (non-reducing conditions)
    Lane 2 : Mouse IgG2a kappa (reducing conditions)

    Lysates/proteins at 0.02 µg per lane.
  • ELISA analysis of mouse immunoglobulins labeling the Fab region of mouse IgG2a using ab190463 at 200 ng/ml, 50 ng/ml, or 10 ng/ml. The plate was coated with 50 ng/well of different immunoglobulins. No cross reactivity with IgG2aλ, IgG1, IgG3, IgM, IgA, IgE, Human IgG, rat IgG, or goat IgG was detected. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • ELISA analysis of mouse IgG2a using ab190463 at different concentrations. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

References

ab190463 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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