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Full length protein corresponding to Mouse IgG2a.
Our Abpromise guarantee covers the use of ab190463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use a concentration of 0.5 - 2 µg/ml.|
|IP||Use at an assay dependent concentration.|
|ELISA||Use a concentration of 0.005 - 0.2 µg/ml.|
|WB||Use a concentration of 0.1 - 0.5 µg/ml.
Non-reducing conditions only
|IHC-P||Use a concentration of 0.5 - 2 µg/ml.|
ELISA analysis of mouse immunoglobulins labeling the Fab region of mouse IgG2a using ab190463 at 200 ng/ml, 50 ng/ml, or 10 ng/ml. The plate was coated with 50 ng/well of different immunoglobulins. No cross reactivity with IgG2aλ, IgG1, IgG3, IgM, IgA, IgE, Human IgG, rat IgG, or goat IgG was detected. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA analysis of mouse IgG2a using ab190463 at different concentrations. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ab190463 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"