The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 106 cells. ab91361 is a negative isotype control
References for Mouse IgG2a [ICIGG2A] - Isotype Control (ab91361)
This product has been referenced in:
Abo Al Hayja M et al. Reduced expression of peroxisome proliferator-activated receptor alpha in BAL and blood T cells of non-löfgren's sarcoidosis patients. J Inflamm (Lond)12:28 (2015).
Read more (PubMed: 25969669) »
Tashima Y & Stanley P Antibodies that detect O-linked ß-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteins. J Biol Chem289:11132-42 (2014).
Read more (PubMed: 24573683) »