Mouse IgG2a, kappa [MOPC-173] (Biotin) - Isotype Control (ab18454)

Q&A (1)

Overview

  • Product name
    Mouse IgG2a, kappa [MOPC-173] (Biotin) - Isotype Control
    See all Mouse isotype controls
  • Conjugation
    Biotin
  • Specificity
    ab18454 MOPC-173 immunoglobulin has unknown specificity. ab18454 MOPC-173 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues.
  • Tested applications
    Suitable for: Flow Cytmore details
  • General notes


    ab18454 MOPC-173 immunoglobulin is useful as an isotype-matched control for Western blotting, immunoprecipitation, immunohistochemical staining, and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab18454 antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
  • Isotype control notes
    ab18454 MOPC-173 immunoglobulin is useful as an isotype-matched control for Western blotting, immunoprecipitation, immunohistochemical staining, and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
  • Clonality
    Monoclonal
  • Clone number
    MOPC-173
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Suitable for surface or intracellular staining.

References

ab18454 has not yet been referenced specifically in any publications.

Publishing research using ab18454? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1 Q&A

10
Votes: Highest First

Dear Abcam,
I would like to ask a question about which control IgG I need to use for
28-14-8 (biotin) (#ab25237)
I want to use same isotype with biotin conjugated antibody and I found that ab 128976, which is biotinylated
Goat anti-...

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Thank you for contacting us.

I wouldn't recommend using ab128976 as an isotype control with ab25237 since ab128976 is raised in a goat and it needs to match the primary antibody you're using.

I would recommend ab18454 as an isotype...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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