Mouse IgG2a [MOPC-173] Isotype Control- ChIP Grade (ab18413)

Overview

  • Product name
    Mouse IgG2a [MOPC-173] Isotype Control- ChIP Grade
    See all Mouse isotype controls
  • Specificity
    This Balb/c myeloma derived clone has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, Flow Cyt, IP, WB, ChIP/Chip, ChIPmore details

Properties

  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ChIP/Chip Use at an assay dependent concentration. PubMed: 18636108
ChIP Use at an assay dependent concentration. PubMed: 18636108

Mouse IgG2a [MOPC-173] Isotype Control- ChIP Grade images

  • ChIP of ΔNp73 binding with SBE. The relative amount of ΔNp73 associated DNA as pulled down with an antibody directed against HA, is represented as a fold enrichment compared to pull-down with IgG (background, ab18413). Gene enrichment was quantified by qPCR using primers specific for the promoter regions of A) PAI-1, B) Col1a1 and C) p21WAF within the SBEs. Primers specific for PTEN (D) were used as a positive control. Pulldown antibody is shown on the x-axis, with y-axis showing fold enrichment ± SEM. * represents p-value>0.05.

References for Mouse IgG2a [MOPC-173] Isotype Control- ChIP Grade (ab18413)

This product has been referenced in:
  • Wu T  et al. Expression of Ferritin Light Chain (FTL) Is Elevated in Glioblastoma, and FTL Silencing Inhibits Glioblastoma Cell Proliferation via the GADD45/JNK Pathway. PLoS One 11:e0149361 (2016). IP . Read more (PubMed: 26871431) »
  • Kincaid AE  et al. Specificity, Size, and Frequency of Spaces That Characterize the Mechanism of Bulk Transepithelial Transport of Prions in the Nasal Cavities of Hamsters and Mice. J Virol 90:8293-301 (2016). Read more (PubMed: 27384659) »

See all 17 Publications for this product

Product Wall

Thank you for your reply.

They are the same antibody (same clone), just that ab18449 has been screened for low endotoxin levels for use with cell culture.

I hope this information helps. Please contact us with any other questions.

Thank you for contacting us.

The anti-IL6 antibody ab9324 is a mouse monoclonal IgG2a. Therefore, you will need to use a mouse monoclonal IgG2a isotype control.

Therefore, you should use the mouse control IgG2a ab18413 and NOT the ...

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Thank you for contacting us.

I can confirm that ab18413 is isotype IgG2a, therefore is only suitable for one of the primary antibodies the customer is using. I have asked our products team to update the datasheet.

Apologies for an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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