Recombinant

Anti-Mouse IgG2b antibody [RM108] (ab190482)

Overview

  • Product name
    Anti-Mouse IgG2b antibody [RM108]
  • Description
    Rabbit monoclonal [RM108] to Mouse IgG2b
  • Host species
    Rabbit
  • Specificity
    ab190482 reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The Fc region of ab190482 has been engineered to eliminate the binding of Fc receptor.
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IP, ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Full length protein corresponding to Mouse IgG2b.
    Database link: P01867

  • Positive control
    • Mouse IgG2b.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 48% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab190482 is purified from an animal origin–free and protein-free culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    RM108
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use a concentration of 0.5 - 2 µg/ml.
IP Use at an assay dependent concentration.
ELISA Use a concentration of 0.005 - 0.2 µg/ml.
WB Use a concentration of 0.1 - 0.5 µg/ml.
IHC-P Use a concentration of 0.5 - 2 µg/ml.

Target

  • Database links
  • Alternative names
    • gamma2b antibody
    • Ig gamma-2B chain C region antibody
    • IgG2b antibody
    • Igh-3 antibody
    • Ighg2b antibody
    • immunoglobulin heavy constant gamma 2B antibody
    see all

Images

  • All lanes : Anti-Mouse IgG2b antibody [RM108] (ab190482) at 0.2 µg/ml

    Lane 1 : Mouse IgG2b, non-reduced
    Lane 2 : Mouse IgG2b, reduced

    Lysates/proteins at 0.02 µg per lane.


    ab190482 reacted to non-reduced IgG2b (~150 kDa) much stronger than the reduced γ2b(~50 kDa).

References

ab190482 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab190482.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up