The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 2µg for 106 cells.
ab91366 is a negative isotype control
References for Mouse IgG2b [PLPV219] - Isotype Control (ab91366)
This product has been referenced in:
Abo Al Hayja M et al. Reduced expression of peroxisome proliferator-activated receptor alpha in BAL and blood T cells of non-löfgren's sarcoidosis patients. J Inflamm (Lond)12:28 (2015).
Read more (PubMed: 25969669) »
Carilho Torrao RB et al. Healthy ageing and depletion of intracellular glutathione influences T cell membrane thioredoxin-1 levels and cytokine secretion. Chem Cent J7:150 (2013).
Read more (PubMed: 24007191) »