Mouse IgG2b [PLPV219] - Isotype Control (ab91366)


  • Product nameMouse IgG2b [PLPV219] - Isotype Control
    See all Mouse isotype controls
  • Specificityab91366 was produced against a synthetic hapten, which is normally not present in Humans or animals. Therefore, ab91366 does not react with any antigen of Human or animal cells.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Immunogen

    Vegetal protein


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberPLPV219
  • IsotypeIgG2b
  • Research areas
  • Alternative names
    • Mouse Isotype Control


Our Abpromise guarantee covers the use of ab91366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells. ab91366 is a negative isotype control

Mouse IgG2b [PLPV219] - Isotype Control images

  • Overlay histogram showing HeLa cells stained with ab54944 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54944, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Mouse IgG2b [PLPV219] - Isotype Control (ab91366)

This product has been referenced in:
  • Abo Al Hayja M  et al. Reduced expression of peroxisome proliferator-activated receptor alpha in BAL and blood T cells of non-löfgren's sarcoidosis patients. J Inflamm (Lond) 12:28 (2015). Flow Cyt ; Human . Read more (PubMed: 25969669) »
  • Carilho Torrao RB  et al. Healthy ageing and depletion of intracellular glutathione influences T cell membrane thioredoxin-1 levels and cytokine secretion. Chem Cent J 7:150 (2013). Flow Cyt . Read more (PubMed: 24007191) »

See all 2 Publications for this product

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Regarding PPAR-delta ab:
I think ab58137 ( as you suggested would be the best antibody to try as it has been tested in flow cyt and using the same secondary ab (ab96879) would be fine. Howeve...

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