|Sample type||Average %||Range|
|Cell culture supernatant||113.6||104% - 123%|
|Serum||122.4||113% - 132%|
|Plasma||112.3||77% - 140%|
Abcam’s IL-1 alpha (Interleukin-1 alpha) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-1 alpha in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|5X Assay Diluent D||1 x 15ml|
|500X HRP-Streptavidin Concentrate||1 x 200µl|
|Biotinylated anti-Mouse IL-1 alpha||2 vials|
|IL-1 alpha Microplate (12 x 8 wells)||1 unit|
|Recombinant Mouse IL-1 alpha Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab113344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Representative Standard Curve using ab113344
ab113344 has not yet been referenced specifically in any publications.