Mouse IL-1 beta ELISA Kit (Interleukin-1 beta) (ab100705)

Overview

  • Product nameMouse IL-1 beta ELISA Kit (Interleukin-1 beta)
    See all IL1 beta kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    2.74 pg/ml - 2000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 102.34 90% - 111%
    Tissue Extracts 98.26 89% - 109%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IL-1 beta Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-1 beta in cell lysates and tissue lysates.


    This assay employs an antibody specific for mouse IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    For higher sensitivity and reproducibility in only 90 minutes, we recommend Mouse IL-1 beta ELISA Kit (ab197742) from our SimpleStep ELISA® range.


     

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similaritiesBelongs to the IL-1 family.
  • DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • Alternative names
    • Catabolin
    • H1
    • Hematopoietin 1
    • IFN beta inducing factor
    • IL 1
    • IL 1 beta
    • IL 1B
    • IL-1 beta
    • IL1
    • IL1 BETA
    • IL1B
    • IL1B_HUMAN
    • IL1F2
    • Interleukin 1 beta
    • Interleukin 1 beta precursor
    • Interleukin-1 beta
    • LAF
    • OAF
    • Osteoclast activating factor
    • Preinterleukin beta
    • Pro interleukin 1 beta
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab100705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-1 beta ELISA Kit (Interleukin-1 beta) images

  • Dilution curves of mouse IL-1 beta (ab100705) and NIBSC standard (93/668). One ng of standard mouse IL-1 beta corresponds to 34.6 (+/-2.1) AU NIBSC 93/668. Background signal subtracted (duplicates; +/- SD).

  • IL-1 beta measured in mouse tissue lysates (0.02-0.3 mg x mL-1 protein tested, data expressed per

    mg of extracted protein; duplicates +/- SD).

  • IL-1 beta measured in lysates from control cells, or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297), with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours (duplicates +/- SD).

  • Representative standard curve using ab100705

Protocols

References for Mouse IL-1 beta ELISA Kit (Interleukin-1 beta) (ab100705)

ab100705 has not yet been referenced specifically in any publications.

Product Wall

GOOD

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Sample: Mouse Cell (Homogenised lung tissue)
Specification: Homogenised lung tissue
Type of Kit Used: ELISA
Positive control: Virally infected lung tissue.
Did you use the Abcam Kit protocol?: Yes
Additional data
Additional Notes: Overall, a good kit.
Username

Abcam user community

Verified customer

Submitted Jan 27 2016



It is possible to use the non-lysate kit ab100704 (IL-1 beta (Interleukin-1 beta) Mouse ELISA Kit) and use your own lysis buffers (non-denaturing, with a mild detergent like RIPA/NP40/Triton X). The cell lysate kit (ab100705) contains this bu...

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ıl1beta mouse elisa kit ab100705

Average Good 4/5 (Ease of Use)
Abreviews
This kit ( ab100705 ) is not suitable for the detection of il1 beta levels from the serum of mice. We collected blood from ApoE-/- mice that are on high fat diet for 3 months in addition to ApoE-/- mice fed with chow diet. In none of these serum samples we obtained a promising result. On the other hand, the same kit detected il1beta from liver lysates as kit suggested.
Username

Abcam user community

Verified customer

Submitted Jun 29 2015

Gracias por contactarnos.


He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas espec&iacu...

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Thank you for your reply.


Because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromi...

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Thank you for your patience.

I am still waiting on more information on ab108866.

But I am happy to provide the following information about ab100704 and ab100705:

The antibody pair used in the Mouse IL-1 beta Sandwich ELISA...

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Abreview for IL1 beta Mouse ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Mouse Purified protein (Placenta tissue protein)
Specification: Placenta tissue protein
Amount of Sample: 0.4 µg
Type of Kit Used: ELISA
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Bar-1, control
Bar-2, LPS
Bar-3, Surfactant Protein-A
Bar-4, LPS + SP-A
Username

Dr. Varkha Agrawal

Verified customer

Submitted Sep 26 2012

Thank you for your call yesterday and for your patience while I've been in touch with the lab regarding your enquiry.

We are still looking into the question of what kind of tissue ab100705 was tested with, and I will be back in touch with thi...

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Thank you for your email.
I can confirm that this kit will recognize the mature form (aa 118-269) of IL1beat.
Since the pro IL1beta also contains this amino acid sequence, I would expect the kit to also detect the pro-form. This has not been ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"