Mouse IL-1 beta ELISA kit (Interleukin-1 beta) (ab108866)


  • Product nameMouse IL-1 beta ELISA kit (Interleukin-1 beta)
    See all IL1 beta kits
  • Detection methodColorimetric
  • Precision
    Sample n Mean SD CV%
    Overall 4.3%
    Sample n Mean SD CV%
    Overall 7.5%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.016 ng/ml
  • Range
    0.016 ng/ml - 1 ng/ml
  • Recovery

    98 %

  • Assay time
    5h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IL-1 beta (Interleukin-1 beta) mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse IL-1 beta concentrations in plasma, serum and cell culture supernatants.

    An IL-1 beta specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-1 beta specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-1 beta captured in plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similaritiesBelongs to the IL-1 family.
  • DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • Alternative names
    • Catabolin
    • H1
    • Hematopoietin 1
    • IFN beta inducing factor
    • IL 1
    • IL 1 beta
    • IL 1B
    • IL-1 beta
    • IL1
    • IL1 BETA
    • IL1B
    • IL1B_HUMAN
    • IL1F2
    • Interleukin 1 beta
    • Interleukin 1 beta precursor
    • Interleukin-1 beta
    • LAF
    • OAF
    • Osteoclast activating factor
    • Preinterleukin beta
    • Pro interleukin 1 beta
    see all
  • Database links


Our Abpromise guarantee covers the use of ab108866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Mouse IL-1 beta ELISA kit (Interleukin-1 beta) images

  • Representative Standard Curve using ab108866


References for Mouse IL-1 beta ELISA kit (Interleukin-1 beta) (ab108866)

ab108866 has not yet been referenced specifically in any publications.

Product Wall

Gracias por contactarnos.

He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas espec&iacu...

Read More

Thank you for your reply.

Because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromi...

Read More

Thank you for contacting us. Please be advised that we have not done any test with p-NPP substrate so we will not be able to provide any data regarding this. We have only tested this Kit using the reagent mentioned on the KIT datasheet. Regardin...

Read More

Thank you very much for contacting us. The standard we have provided in the KIT is 2 ng/ml; which is lyophilized. We recommend adding 2 ml of Diluent Concentrate (10X) in the IL1 beta standard vial which will give final conc. of IL1 beta 1ng/ml. ...

Read More