Overview

  • Product name
    Mouse IL-10 ELISA kit (Interleukin-10)
    See all IL10 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Sample type
    Cell culture supernatant, Serum, Plasma, Tissue
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.03 ng/ml
  • Range
    0.031 ng/ml - 2 ng/ml
  • Recovery

    99.5 %

  • Assay time
    5h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IL-10 (Interleukin-10) Mouse in vitro ELISA (enzyme-linked immunosorbent assay) kit is designed for the quantitative measurement of IL-10 in plasma, serum, tissue extracts and cell culture supernatants.

    An IL-10 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-10 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-10 captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Function
    Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificity
    Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similarities
    Belongs to the IL-10 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • CSIF
    • Cytokine synthesis inhibitory factor
    • GVHDS
    • IL 10
    • IL-10
    • IL10
    • IL10_HUMAN
    • IL10A
    • Interleukin 10
    • Interleukin-10
    • Interleukin10
    • MGC126450
    • MGC126451
    • T cell growth inhibitory factor
    • TGIF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-10 ELISA kit (Interleukin-10) images

  • Representative Standard Curve using ab108870

Protocols

References for Mouse IL-10 ELISA kit (Interleukin-10) (ab108870)

This product has been referenced in:
  • Rajan TS  et al. The secretome of periodontal ligament stem cells from MS patients protects against EAE. Sci Rep 6:38743 (2016). ELISA ; Mouse . Read more (PubMed: 27924938) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry which has been forwarded to the scientific support team.

I can confirm that these three ELISA kits ab108870 ab100747 and ab100697 have been tested on mouse plasma and serum samples. (We would not recommend to test ...

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Merci de nous avoir contactés.

L'anticorps anti-IL10 du kit ab108870 est un polyclonal de lapin biotinylé dont l'immunogène est la séquence complète d'IL10 de souris.
La référence catalogue ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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