Overview

  • Product nameMouse IL-11 ELISA Kit (Interleukin-11)
    See all IL11 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 1 ng/ml
  • Range
    1.85 ng/ml - 450 ng/ml
  • Recovery

    99 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 77.35 70% - 87%
    Serum 102.4 92% - 111%
    Plasma 117.3 95% - 113%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IL-11 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-11 in serum, plasma and cell culture supernatants. (Mouse IL-11 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for mouse IL-11 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-11 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-11 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-11 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionDirectly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Adipogenesis inhibitory factor
    • AGIF
    • IL 11
    • IL-11
    • Il11
    • IL11_HUMAN
    • Interleukin 11
    • Interleukin-11
    • Interleukin11
    • Oprelvekin
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-11 ELISA Kit (Interleukin-11) images

  • Representative standard curve using ab100698.

Protocols

References for Mouse IL-11 ELISA Kit (Interleukin-11) (ab100698)

ab100698 has not yet been referenced specifically in any publications.

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