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|Sample type||Average %||Range|
|Cell culture supernatant||137.2||115% - 150%|
|Plasma||128.5||105% - 148%|
Abcam’s IL-15 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-15 in plasma and cell culture supernatants. (Mouse IL-15 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for mouse IL-15 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-15 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-15 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-15 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Mouse IL-15 in normal serum with ab100701, only in serum spiked with Mouse IL-15.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|300X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|5X Assay Diluent D||1 x 15ml|
|Biotinylated anti-mouse IL-15||2 vials|
|IL-15 Microplate (12 x 8 wells)||1 unit|
|Recombinant Mouse IL-15 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|