Overview

  • Product name
    Mouse IL-17 ELISA Kit (Interleukin-17)
    See all IL17 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 6 pg/ml
  • Range
    6.1 pg/ml - 600 pg/ml
  • Recovery

    99 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 99.62 85% - 105%
    Serum 101.38 87% - 107%
    Plasma 97.47 84% - 103%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IL-17 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-17 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse IL-17 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-17 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    700X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-mouse IL-17 (lyophilized) 2 vials
    IL-17 Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse IL-17 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • Tissue specificity
    Restricted to activated memory T-cells.
  • Sequence similarities
    Belongs to the IL-17 family.
  • Post-translational
    modifications
    Found both in glycosylated and nonglycosylated forms.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • CTLA 8
    • CTLA-8
    • CTLA8
    • Cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL 17
    • IL 17A
    • IL-17
    • IL-17A
    • IL17_HUMAN
    • Il17a
    • Interleukin 17A
    • Interleukin-17A
    • Interleukin17
    • Interleukin17A
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100702

  • Representative standard curve using ab100702

Protocols

References

This product has been referenced in:
  • Dietert K  et al. mCLCA3 modulates IL-17 and CXCL-1 induction and leukocyte recruitment in murine Staphylococcus aureus pneumonia. PLoS One 9:e102606 (2014). Read more (PubMed: 25033194) »
  • Kotla S  et al. The transcription factor CREB enhances interleukin-17A production and inflammation in a mouse model of atherosclerosis. Sci Signal 6:ra83 (2013). Read more (PubMed: 24045154) »

See all 2 Publications for this product

Customer reviews and Q&As

When reconstituting your standard, we suggest that after it has been thoroughly defrosted, spin the vial down, reconstitute with assay diluent and then spin down again before use.
When making your standard dilutions, we recommend keeping everythin...

Read More

Thank you for contacting us.
This ELISA kits is specific to IL17A. It does not recognize IL-17B, C, D or F.
Please let me know if you have any questions or comments.

Gracias por contactarnos.


He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas espec&iacu...

Read More

Thank you for your inquiry.

Unfortunately, for the antibodies in IL17 Mouse ELISA Kit ab100702 epitope mapping has not been performed, so we do not know the exact region the antibodies are binding to the mouse IL-17A protein. However, I can ...

Read More

Following up my previous message, here are some recommendations for preparing cell and tissue lysates for use with this kit, though they are not specific for oviducts or lymph nodes: 1) Avoid using SDS or other strongly denaturing detergents. I...

Read More

Thank you for contacting us. Can you please reply with a few details of the trouble you are having with the standard curve? For a positive control, I suggest isolating lymph nodes from a mouse, if possible. For the oviduct homogenate pr...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up