Overview

  • Product name
    Mouse IL-17B ELISA Kit (Interleukin-17B)
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 12 pg/ml
  • Range
    16.46 pg/ml - 12000 pg/ml
  • Recovery

    > 100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 149.4 139% - 161%
    Serum 118.9 104% - 128%
    Plasma 129.3 92% - 155%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IL-17B Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-17B in serum (mouse IL-17B concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse IL-17B coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17B present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-17B antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17B bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    5X Assay Diluent D 1 x 15ml
    Biotinylated anti-mouse IL-17B 2 vials
    IL-17B Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse IL-17B Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1.
  • Tissue specificity
    Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.
  • Sequence similarities
    Belongs to the IL-17 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Cytokine like protein Zcyto7
    • Cytokine Zcyto7
    • IL 17B
    • IL 20
    • IL-17B
    • IL-20
    • IL17B
    • IL17B_HUMAN
    • IL20
    • Interleukin 17 beta
    • Interleukin 17B
    • Interleukin 20
    • Interleukin-17B
    • Interleukin-20
    • Neuronal interleukin 17 related factor
    • Neuronal interleukin-17-related factor
    • NIRF
    • ZCYTO7
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100703

Protocols

References

ab100703 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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