Mouse IL-2 ELISA Kit (Interleukin-2) (ab100706)

Overview

  • Product nameMouse IL-2 ELISA Kit (Interleukin-2)
    See all IL2 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 1 pg/ml
  • Range
    0.625 pg/ml - 40 pg/ml
  • Recovery

    98 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 100.34 89% - 110%
    Serum 97.56 85% - 104%
    Plasma 98.34 86% - 106%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IL-2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-2 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionProduced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • Involvement in diseaseNote=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
  • Sequence similaritiesBelongs to the IL-2 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Aldesleukin
    • IL 2
    • IL-2
    • IL2
    • IL2_HUMAN
    • Interleukin 2
    • Interleukin-2
    • Interleukin2
    • Involved in regulation of T cell clonal expansion
    • Lymphokine
    • T cell growth factor
    • T-cell growth factor
    • TCGF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-2 ELISA Kit (Interleukin-2) images

  • Representative standard curve using ab100706

  • Representative standard curve using ab100706

Protocols

References for Mouse IL-2 ELISA Kit (Interleukin-2) (ab100706)

ab100706 has not yet been referenced specifically in any publications.

Product Wall

I have broken down the cross-reactivity information for each kit below:


· ab100549 IL10 Human ELISA Kit
o The capture antibody did not show any cross-reactivity with Murine IL-10 from testing. Thus, this kit is not expected ...

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I can confirm that these kit are suitable for the use with serum samples that have been frozen.

I recommend to not to mix/compare samples thathave been frozen with samples that have not been frozen unless...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"