Overview

  • Product nameMouse IL-2 ELISA Kit (Interleukin-2)
    See all IL2 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 10 170pg/ml 13 7.8%
    Inter-assay
    Sample n Mean SD CV%
    B 8 137pg/ml 8.9 6.4%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    15.6 pg/ml - 500 pg/ml
  • Assay time
    3h 45m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IL-2 (Interleukin-2) mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2 in mouse serum, buffered solutions and cell culture medium.

    A monoclonal antibody specific for IL-2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2 present in the samples.

    This kit will recognize both endogenous and recombinant mouse IL-2.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionProduced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • Involvement in diseaseNote=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
  • Sequence similaritiesBelongs to the IL-2 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Aldesleukin
    • IL 2
    • IL-2
    • IL2
    • IL2_HUMAN
    • Interleukin 2
    • Interleukin-2
    • Interleukin2
    • Involved in regulation of T cell clonal expansion
    • Lymphokine
    • T cell growth factor
    • T-cell growth factor
    • TCGF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-2 ELISA Kit (Interleukin-2) images

  • Representative Standard Curve using ab46096.

Protocols

References for Mouse IL-2 ELISA Kit (Interleukin-2) (ab46096)

ab46096 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab46096.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"