Mouse IL-21 ELISA Kit (Interleukin-21) (ab100707)

Overview

  • Product nameMouse IL-21 ELISA Kit (Interleukin-21)
    See all IL21 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 6 pg/ml
  • Range
    8.19 pg/ml - 2000 pg/ml
  • Recovery

    89 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.65 87% - 109%
    Serum 93.57 83% - 104%
    Plasma 77.38 68% - 87%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IL-21 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-21 in serum, plasma and cell culture supernatants. (Mouse IL-21 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for mouse IL-21 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-21 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-21 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-21 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceA novel cytokine related to IL2 and IL15 was recently identified and designated IL21. IL21 has been found to be a powerful growth factor for naive B cells. The receptor for IL21 (IL21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL21 signaling. Both IL21R and the gc are necessary for the IL21 function. IL21 and its receptor activate JAKSTAT signaling pathway. IL21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL21 plays a role in the proliferation and maturation of NK, B and T cell populations.
  • Cellular localizationSecreted
  • Alternative names
    • Cytokine CX2
    • DN 452A22.7
    • IL 21
    • Interleukin 21
    • Interleukin 21 isoform
    • Interleukin21
    • OTTMUSP00000007838
    • Za11
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-21 ELISA Kit (Interleukin-21) images

  • Representative standard curve using ab100707

  • Representative standard curve using ab100707

Protocols

References for Mouse IL-21 ELISA Kit (Interleukin-21) (ab100707)

ab100707 has not yet been referenced specifically in any publications.

Product Wall


We recommend a 2 fold dilution for plasma samples for use with all three of the mouse ELISA kits.

Thank you for your enquiry.

I can confirm that both the antibodies provided with ab100707 IL21 Mouse ELISA Kit are polyclonal.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"