Mouse IL-23 ELISA Kit (Interleukin-23) (ab119545)

Overview

  • Product name
    Mouse IL-23 ELISA Kit (Interleukin-23)
    See all IL23 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 2.5%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    16 pg/ml
  • Range
    31.3 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 49 41% - 53%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IL-23 (Interleukin-23) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-23 in cell culture supernatant, serum and plasma (citrate).

    IL-23 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated IL-23 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-23 captured on the plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-Mouse IL-23 monoclonal antibody 1 x 70µl
    IL-23 Standard lyophilized (4,000 pg/mL upon reconstitution) 2 vials
    Microplate coated with monoclonal antibody to Mouse IL-23 (12 x 8 wells) 1 unit
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • Research areas
  • Function
    Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • Tissue specificity
    Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).
  • Sequence similarities
    Belongs to the IL-6 superfamily.
  • Developmental stage
    Expressed by newborns dendritic cells.
  • Cellular localization
    Secreted. Secreted upon association with IL12B.
  • Information by UniProt
  • Alternative names
    • IL 23
    • IL 23A
    • IL-23 subunit alpha
    • IL-23-A
    • IL-23p19
    • Il23a
    • IL23A_HUMAN
    • IL23P19
    • Interleukin 23 alpha subunit p19
    • Interleukin-23 subunit alpha
    • Interleukin-23 subunit p19
    • JKA3 induced upon T cell activation
    • P19
    • SGRF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab119545.

     

Protocols

References

ab119545 has not yet been referenced specifically in any publications.

Customer reviews and Q&As



Ab119545 has only been tested with culture supernatant, serum, and citrate plasma, but it may be suitable for use with untested sample types like tissue lysates.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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