Overview

  • Product name
    Mouse IL-3 ELISA Kit (Interleukin-3)
    See all IL3 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 0.2 pg/ml
  • Range
    0.16 pg/ml - 40 pg/ml
  • Recovery

    > 100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 102.4 68% - 132%
    Serum 116.2 72% - 131%
    Plasma 121.9 85% - 142%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IL-3 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-3 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse IL-3 coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    5X Assay Diluent D 1 x 15ml
    Biotinylated anti-mouse antibody IL-3 (lyophilized) 2 vials
    IL-3 Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse IL-3 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
    This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
  • Tissue specificity
    Activated T-cells, mast cells, natural killer cells.
  • Sequence similarities
    Belongs to the IL-3 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Burst promoting activity
    • Colony stimulating factor multiple
    • Eosinophil colony stimulating factor
    • Hematopoietic growth factor
    • IL 3
    • IL-3
    • IL3
    • IL3_HUMAN
    • Interleukin 3
    • Interleukin-3
    • Mast cell growth factor
    • MCGF
    • Multi CSF
    • Multilineage colony stimulating factor
    • Multipotential colony stimulating factor
    • Multipotential colony-stimulating factor
    • P cell stimulating factor
    • P-cell-stimulating factor
    • Thy1 inducing factor
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab113345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab113345.

Protocols

References

This product has been referenced in:
  • Kim J  et al. Aqueous extract of Phragmitis rhizoma ameliorates myelotoxicity of docetaxel in vitro and in vivo. BMC Complement Altern Med 17:393 (2017). Read more (PubMed: 28793897) »

See 1 Publication for this product

Customer reviews and Q&As

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