Overview

  • Product name
    Mouse IL-5 ELISA Kit (Interleukin-5)
    See all IL5 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 1 pg/ml
  • Range
    2.05 pg/ml - 500 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.76 85% - 104%
    Serum 93.44 82% - 102%
    Plasma 95.49 93% - 104%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IL-5 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-5 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for mouse IL-5 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get better reproducibility in only 90 minutes with Mouse IL-5 ELISA Kit (ab204523) from our SimpleStep ELISA® range.


     

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab100711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100711

  • Representative standard curve using ab100711

Protocols

References

ab100711 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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