Mouse IL-7 ELISA Kit (Interleukin-7) (ab100714)


  • Product nameMouse IL-7 ELISA Kit (Interleukin-7)
    See all IL7 kits
  • Detection methodColorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.58 85% - 105%
    Serum 91.59 82% - 103%
    Plasma 92.66 83% - 104%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IL-7 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-7 in serum, plasma and cell culture supernates.

    This assay employs an antibody specific for mouse IL-7 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • RelevanceIL7 is a cytokine important for B and T cell development. It and the hepatocyte growth factor form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL7 is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. It can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that IL7 plays an essential role in lymphoid cell survival.
  • Cellular localizationSecreted
  • Alternative names
    • IL 7
    • IL7 nirs variant 6
    • Interleukin 7
    • Interleukin7
  • Database links


Our Abpromise guarantee covers the use of ab100714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-7 ELISA Kit (Interleukin-7) images

  • Representative standard curve using ab100714

  • Representative standard curve using ab100714


References for Mouse IL-7 ELISA Kit (Interleukin-7) (ab100714)

ab100714 has not yet been referenced specifically in any publications.

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Thank you for contacting Abcam regarding ab100714.

The IL7 Mouse ELISA Kit will detect soluble IL-7. As this kit was not validated with cell or tissue lysate samples, we do not know if the kit will also detect IL-7 bound to its membrane...

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