Overview

  • Product nameMouse IL-9 ELISA Kit (Interleukin-9)
    See all IL9 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.1 ng/ml
  • Range
    0.082 ng/ml - 60 ng/ml
  • Recovery

    90 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 105.4 98% - 121%
    Serum 83.28 75% - 96%
    Plasma 82.16 74% - 95%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IL-9 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-9 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse IL-9 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionSupports IL-2 independent and IL-4 independent growth of helper T-cells.
  • Sequence similaritiesBelongs to the IL-7/IL-9 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Cytokine P40
    • Homolog of mouse T cell and mast cell growth factor 40
    • HP40
    • IL-9
    • Il9
    • IL9_HUMAN
    • Interleukin 9
    • Interleukin-9
    • Mast cell growth factor
    • MCGF
    • Megakaryoblast growth factor
    • p40 cytokine
    • T cell growth factor 3
    • T cell growth factor p40
    • T-cell growth factor P40
    • TCGF 3
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-9 ELISA Kit (Interleukin-9) images

  • Representative standard curve using ab100715

  • Representative standard curve using ab100715

Protocols

References for Mouse IL-9 ELISA Kit (Interleukin-9) (ab100715)

ab100715 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab100715.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"