Overview

  • Product name
    Mouse IL10 ELISA Set (without plates)
    See all IL10 kits
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    31.25 pg/ml - 1000 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IL-10 Murine in vitro ELISA Set is designed for the quantitative measurement of Murine IL-10 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    The murine IL10 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    This ELISA Set is intended to measure murine IL10 in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

    Recognizes both natural and recombinant Murine IL10

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Reagents

Properties

  • Function
    Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificity
    Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similarities
    Belongs to the IL-10 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • CSIF
    • Cytokine synthesis inhibitory factor
    • IL-10
    • IL10
    • IL10_HUMAN
    • Interleukin 10
    • Interleukin-10
    • TGIF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab47599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Mouse IL10 ELISA Set (without plates) images

    • Murine IL-10 standard curve ranging from 31.25 to 1000 pg/mL

    References for Mouse IL10 ELISA Set (without plates) (ab47599)

    ab47599 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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