Mouse Inflammation Antibody Array - Membrane (40 Targets) (ab133999)
Overview
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Product name
Mouse Inflammation Antibody Array - Membrane (40 Targets)
See all Inflammation Antibody Array antibody arrays -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Semi-quantitative -
Species reactivity
Reacts with: Mouse -
Product overview
ab133999 is for simultaneous detection of 40 Mouse inflammatory factors. Suitable for all sample types.
Targets: BLC, CD30L, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CSF, IFN-gamma, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, I-TAC, KC/CXCL1, Leptin/OB, LIX, Lymphotactin, MCP-1, MCSF, MIG, MIP-1 alpha, MIP-1 gamma, RANTES, SDF-1, TCA-3, TECK, TIMP-1, TIMP-2, TNF-alpha, sTNF RI, sTNF-RII
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
Learn more about membrane antibody arrays
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Notes
A table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Multiplex Protein Detectionmore details
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 4 Membranes 1 x 8 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Biotin-Conjugated Anti-Cytokines 2 vials 4 vials Inflammation Antibody Array Membranes 4 units 8 units Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Multiplex Protein Detection |
Use at an assay dependent concentration.
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Notes |
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Multiplex Protein Detection
Use at an assay dependent concentration. |
Images
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab133999 has been referenced in 16 publications.
- Borza CM et al. DDR1 contributes to kidney inflammation and fibrosis by promoting the phosphorylation of BCR and STAT3. JCI Insight 7:N/A (2022). PubMed: 34941574
- Matyśniak D et al. P2X7 receptor: the regulator of glioma tumor development and survival. Purinergic Signal 18:135-154 (2022). PubMed: 34964926
- Lelliott EJ et al. NKG7 Enhances CD8+ T Cell Synapse Efficiency to Limit Inflammation. Front Immunol 13:931630 (2022). PubMed: 35874669
- Pontes-Quero GM et al. Modulation of Inflammatory Mediators by Polymeric Nanoparticles Loaded with Anti-Inflammatory Drugs. Pharmaceutics 13:N/A (2021). PubMed: 33672354
- Kim YS et al. The adipokine Retnla deficiency increases responsiveness to cardiac repair through adiponectin-rich bone marrow cells. Cell Death Dis 12:307 (2021). PubMed: 33753732