Mouse Interferon gamma ELISA Kit (ab46081)

Overview

  • Product nameMouse Interferon gamma ELISA Kit
    See all Interferon gamma kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 20 315.4pg/ml 14.5 4.6%
    B 19 588.6pg/ml 19.1 4.6%
    Inter-assay
    Sample n Mean SD CV%
    A 9 128.8pg/ml 7.4 5.7%
    B 10 546.9pg/ml 25.2 4.6%
  • Tests
    1 x 96 well plate
  • Sample type
    Milk, Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    31.25 pg/ml - 1000 pg/ml
  • Assay time
    3h 45m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IFN gamma mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IFN gamma in mouse serum, buffered solutions and cell culture medium.

    A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples.

    This kit will recognize both endogenous and recombinant mouse IFN gamma.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceMammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
  • Alternative names
    • IFG
    • IFI
    • IFN gamma
    • IFN Immune
    • IFNG
    • Interferon gamma
    • Interferon Gamma Precursor
    • Macrophage Activating Factor
    • MAF
    • T Cell Interferon
    • Type II Interferon
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab46081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse Interferon gamma ELISA Kit images

  •  Representative Standard Curve using ab46081

Protocols

References for Mouse Interferon gamma ELISA Kit (ab46081)

ab46081 has not yet been referenced specifically in any publications.

Product Wall

Gracias por contactarnos.


He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas espec&iacu...

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Unfortunately, this kit is a ELISpot assay dedicated to detect IFNg secreting cells and not appropriate for serum.

This product will work for your customer's samples:

http://www.abcam.com/index.html?datasheet=46081 (or use ...

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Thank you for contacting us.

The HRP-streptavidin is available in catalogue. Please buy the product ab96467.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or informatio...

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Thank you for contacting us. We still had your email address wrong. Now it is fine, you should receiving my emails.

To let you know I have requested information from the lab regarding the HRP-strepatavidin conjugateand now waiting a reply fr...

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I have requested information from the lab and now waiting a reply.

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Thank you for contacting us.

I have had the chance to speak with the lab regarding your inquiry.Regrettably, the standards includedwithin the Interferon gamma Mouse ELISA kit are not correlated with Biological activity Unit; these tests ha...

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Abreview for Interferon gamma Mouse ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Mouse Serum (Plasma)
Specification: Plasma
Amount of Sample: 10 µg
Type of Kit Used: ELISA
Positive control: C57Bl/6 male mice 8 weeks of age were treated with LPS IP 1mg/kg (0111:B4). After 4 hours blood was collected in EDTA coated tubes. 1:10 dilutions of plasma were made with standard dilutend and used for ELISA. All samples (1:10 diluted) were in range of the standard curve.
Did you use the Abcam Kit protocol?: Yes
Username

Mr. Thomas Milsom

Verified customer

Submitted Feb 24 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"