Mouse Interferon gamma ELISA Kit (IFNG) (ab100690)

Overview

  • Product nameMouse Interferon gamma ELISA Kit (IFNG)
    See all Interferon gamma kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    2.74 pg/ml - 2000 pg/ml
  • Recovery

    92 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 91.95 84% - 104%
    Tissue Extracts 93.47 83% - 103%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Interferon gamma Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Interferon gamma in cell lysate and tissue lysate.

    This assay employs an antibody specific for Mouse Interferon gamma coated on a 96- well plate. Standards and samples are pipetted into the wells and Interferon gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Interferon gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Interferon gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceMammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
  • Alternative names
    • IFG
    • IFI
    • IFN gamma
    • IFN Immune
    • IFNG
    • Interferon gamma
    • Interferon Gamma Precursor
    • Macrophage Activating Factor
    • MAF
    • T Cell Interferon
    • Type II Interferon
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse Interferon gamma ELISA Kit (IFNG) images

  • Representative Standard Curve

Protocols

References for Mouse Interferon gamma ELISA Kit (IFNG) (ab100690)

ab100690 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"