Overview

  • Product name
    Mouse Interferon gamma ELISA Kit (IFNG)
    See all Interferon gamma kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    2.74 pg/ml - 2000 pg/ml
  • Recovery

    92 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 91.95 84% - 104%
    Tissue Extracts 93.47 83% - 103%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Interferon gamma Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Interferon gamma in cell lysate and tissue lysate.

    This assay employs an antibody specific for Mouse Interferon gamma coated on a 96- well plate. Standards and samples are pipetted into the wells and Interferon gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Interferon gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Interferon gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    2x Cell lysate buffer 1 x 5ml
    440X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    5X Sample Diluent Buffer 1 x 10ml
    Biotinylated anti-Mouse Interferon gamma 2 vials
    Interferon gamma Microplate (12 strips x 8 wells) 1 unit
    Recombinant Mouse Interferon gamma Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
  • Alternative names
    • IFG
    • IFI
    • IFN gamma
    • IFN Immune
    • IFNG
    • Interferon gamma
    • Interferon Gamma Precursor
    • Macrophage Activating Factor
    • MAF
    • T Cell Interferon
    • Type II Interferon
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve

Protocols

References

This product has been referenced in:
  • Rajan TS  et al. The secretome of periodontal ligament stem cells from MS patients protects against EAE. Sci Rep 6:38743 (2016). ELISA ; Mouse . Read more (PubMed: 27924938) »

See 1 Publication for this product

Customer reviews and Q&As

Gracias por contactarnos.


He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas espec&iacu...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up