Mouse IP10 ELISA Kit (ab100675)
Key features and details
- Sensitivity: 15 pg/ml
- Range: 8.2 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IP10 ELISA Kit
See all IP10 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 15 pg/ml -
Range
8.2 pg/ml - 2000 pg/ml -
Recovery
97 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 98.86 88% - 107% Serum 97.68 86% - 106% Plasma 96.42 85% - 104% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s IP10 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IP10 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IP10 coated on a 96- well plate. Standards and samples are pipetted into the wells and IP10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IP10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IP10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Mouse IP-10 ELISA Kit (CXCL10) (ab214563) from our SimpleStep ELISA® range.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 80x HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Mouse IP10 2 vials IP10 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IP10 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsCXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Interferon gamma induced factor MOB1, mouse, homolog of
- Interferon gamma induced protein 10
- 10 kDa interferon gamma induced protein
see all -
Database links
- Entrez Gene: 15945 Mouse
- SwissProt: P17515 Mouse
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab100675 has been referenced in 1 publication.
- Zaheer S et al. Clinical course of myelin oligodendrocyte glycoprotein 35-55 induced experimental autoimmune encephalomyelitis is aggravated by glia maturation factor. Neurochem Int 60:215-9 (2012). PubMed: 22226840