Overview

  • Product nameMouse IP10 ELISA Kit
    See all IP10 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    8.2 pg/ml - 2000 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.86 88% - 107%
    Serum 97.68 86% - 106%
    Plasma 96.42 85% - 104%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IP10 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IP10 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse IP10 coated on a 96- well plate. Standards and samples are pipetted into the wells and IP10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IP10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IP10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionChemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Interferon gamma induced factor MOB1, mouse, homolog of
    • Interferon gamma induced protein 10
    • 10 kDa interferon gamma induced protein
    • 10 kDa interferon gamma-induced protein
    • C X C motif chemokine 10
    • C7
    • Chemokine (C X C motif) ligand 10
    • Chemokine CXC motif ligand 10
    • Crg 2
    • CRG2
    • CXCL10
    • CXCL10(1-73)
    • CXL10_HUMAN
    • Gamma IP10
    • Gamma-IP10
    • gIP 10
    • GIP10
    • IFI10
    • INP 10
    • INP10
    • Interferon activated gene 10
    • Interferon gamma induced cell line
    • Interferon inducible cytokine IP 10
    • Interferon inducible cytokine IP10
    • IP 10
    • IP-10
    • Mob 1
    • MOB1
    • Protein 10 from interferon (gamma) induced cell line
    • SCYB10
    • Small inducible cytokine B10
    • Small inducible cytokine B10 precursor
    • Small inducible cytokine subfamily B (Cys X Cys) member 10
    • Small inducible cytokine subfamily B CXC member 10
    • Small inducible cytokine subfamily B, member 10
    • Small-inducible cytokine B10
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse IP10 ELISA Kit images

  • Representative Standard Curve using ab100675

  • Representative Standard Curve using ab100675

Protocols

References for Mouse IP10 ELISA Kit (ab100675)

ab100675 has not yet been referenced specifically in any publications.

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