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|Sample type||Average %||Range|
|Cell culture supernatant||98.86||88% - 107%|
|Serum||97.68||86% - 106%|
|Plasma||96.42||85% - 104%|
Abcam’s IP10 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IP10 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IP10 coated on a 96- well plate. Standards and samples are pipetted into the wells and IP10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IP10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IP10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Mouse IP-10 ELISA Kit (CXCL10) (ab214563) from our SimpleStep ELISA® range.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|80x HRP-Streptavidin Concentrate||1 x 200µl|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Mouse IP10||2 vials|
|IP10 Microplate (12 x 8 wells)||1 unit|
|Recombinant Mouse IP10 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100675 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"