Overview

  • Product nameMouse KIM1 ELISA Kit (TIM-1)
    See all TIM 1 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Homogenate
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    31.2 pg/ml - 2000 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s mouse KIM-1 (TIM-1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse KIM-1 (TIM-1) in cell culture supernatants, tissue homogenates, serum, plasma (heparin, EDTA) and urine. 


    A KIM-1 (TIM-1) specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and are incubated. A biotinylated detection polyclonal antibody from goat specific for KIM-1 (TIM-1) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse KIM-1 (TIM-1) amount of sample captured in plate.


    For higher sensitivity and reproducibility in only 90 minutes, we recommend Mouse KIM-1 ELISA Kit (TIM1) (ab213477) from our SimpleStep ELISA® range.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionMay play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
  • Tissue specificityWidely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. TIM family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Alternative names
    • CD365
    • HAVCR
    • HAVCR 1
    • HAVcr-1
    • Havcr1
    • Hepatitis A virus cellular receptor 1
    • Kidney injury molecule 1
    • KIM 1
    • KIM-1
    • T cell immunoglobin domain and mucin domain protein 1
    • T cell immunoglobulin mucin family member 1
    • T cell immunoglobulin mucin receptor 1
    • T-cell immunoglobulin and mucin domain-containing protein 1
    • T-cell membrane protein 1
    • TIM
    • TIM-1
    • TIM1
    • TIMD 1
    • TIMD-1
    • TIMD1
    • TIMD1_HUMAN
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab119596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse KIM1 ELISA Kit (TIM-1) images

  • Representative Standard Curve for ab119596

Protocols

References for Mouse KIM1 ELISA Kit (TIM-1) (ab119596)

ab119596 has not yet been referenced specifically in any publications.

Product Wall

I have received information regarding

ab119589 Cystatin C Human ELISA Kit
ab119590 Cystatin C Mouse ELISA Kit
ab119596 KIM1 Mouse ELISA Kit
ab119597 KIM1 Rat ELISA Kit
ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit
ab119601 Li...

Read More

Thank you for contacting us.


To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the de...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"