Overview

  • Product name
    Mouse MCP1 ELISA Kit
    See all MCP1 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 3 pg/ml
  • Range
    2.74 pg/ml - 2000 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.29 84% - 104%
    Serum 94.28 83% - 103%
    Plasma 92.79 82% - 102%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s MCP1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MCP1 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse MCP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • MCP1 measured in mouse lysates showing quantity (pg) per microgram of total protein. Values retrieved from 1:100 and 1:1000 dilutions.

  • Representative standard curve using ab100721.

  • Representative standard curve using ab100721.

Protocols

References

This product has been referenced in:
  • Lu HJ  et al. Ruscogenin ameliorates experimental nonalcoholic steatohepatitis via suppressing lipogenesis and inflammatory pathway. Biomed Res Int 2014:652680 (2014). Read more (PubMed: 25136608) »

See 1 Publication for this product

Customer reviews and Q&As

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Ab100721:
Capture Antibody – Mouse Monoclonal
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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