Overview

  • Product nameMouse MCP5 ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.5 pg/ml
  • Range
    0.41 pg/ml - 100 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.34 84% - 105%
    Serum 92.42 81% - 103%
    Plasma 94.14 82% - 104%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s MCP5 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MCP5 in serum, plasma and cell culture supernates.

    This assay employs an antibody specific for mouse MCP5 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MCP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceMCP5 is a member of the b-chemokine family (C-C) of cytokines. This chemokine is strongly chemotactic for human monocytes and murine peritoneal macrophages, but is not active on mouse neutrophils and shows only a weak response on mouse eosinophils at high concentrations
  • Alternative names
    • C C motif chemokine 12
    • Ccl12
    • MCP 1 related chemokine
    • Monocyte chemoattractant protein 5
    • Monocyte chemotactic protein 5
    • Scya12
    • Small inducible cytokine A12
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse MCP5 ELISA Kit images

  • Representative standard curve using ab100723

  • Representative standard curve using ab100723

Protocols

References for Mouse MCP5 ELISA Kit (ab100723)

ab100723 has not yet been referenced specifically in any publications.

Product Wall


We recommend a 2 fold dilution for plasma samples for use with all three of the mouse ELISA kits.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"