Overview

  • Product nameMouse MIG ELISA Kit (CXCL9)
    See all CXCL9 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 3 pg/ml
  • Range
    2.741 pg/ml - 2000 pg/ml
  • Recovery

    83 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 74.58 67% - 81%
    Serum 91.81 80% - 101%
    Plasma 85.55 77% - 93%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s MIG Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIG in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse MIG coated on a 96-well plate. Standards and samples are pipetted into the wells and MIG present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIG bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionCytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • C-X-C motif chemokine 9
    • Chemokine (C-X-C motif) ligand 9
    • Chemokine, CXC motif, ligand 9
    • CMK
    • Crg 10
    • CXCL9
    • CXCL9_HUMAN
    • Gamma interferon induced monokine
    • Gamma-interferon-induced monokine
    • HuMIG
    • MIG
    • Monokine induced by gamma interferon
    • Monokine induced by interferon gamma
    • Monokine induced by interferon-gamma
    • SCYB9
    • Small inducible cytokine B9
    • Small inducible cytokine subfamily B member 9
    • Small-inducible cytokine B9
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse MIG ELISA Kit (CXCL9) images

  • Representative standard curve using ab100725

  • Representative standard curve using ab100725

Protocols

References for Mouse MIG ELISA Kit (CXCL9) (ab100725)

ab100725 has not yet been referenced specifically in any publications.

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