|Sample type||Average %||Range|
|Cell culture supernatant||110.1||102% - 120%|
|Serum||96.1||90% - 108%|
|Plasma||88.7||73% - 108%|
Abcam’s Milk Fat Globule 1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of mouse Milk Fat Globule 1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse Milk Fat Globule 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Milk Fat Globule 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Milk Fat Globule 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Milk Fat Globule 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get better reproducibility in only 90 minutes with Mouse Milk Fat Globule 1 ELISA Kit (ab208981) from our SimpleStep ELISA® range.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|400X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent||1 x 15ml|
|Biotinylated anti-mouse Milk Fat Globule 1||2 vials|
|Milk Fat Globule 1 Microplate (12 x 8 wells)||1 unit|
|Recombinant Mouse Milk Fat Globule 1 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100724 has not yet been referenced specifically in any publications.