• Product name
    Mouse Milk Fat Globule 1 ELISA Kit
    See all Milk Fat Globule 1 kits
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 1 pg/ml
  • Range
    0.823 pg/ml - 600 pg/ml
  • Recovery

    98 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 110.1 102% - 120%
    Serum 96.1 90% - 108%
    Plasma 88.7 73% - 108%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Milk Fat Globule 1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of mouse Milk Fat Globule 1 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse Milk Fat Globule 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Milk Fat Globule 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Milk Fat Globule 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Milk Fat Globule 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    Get better reproducibility in only 90 minutes with Mouse Milk Fat Globule 1 ELISA Kit (ab208981) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Function
    Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.
    Medin is the main constituent of aortic medial amyloid.
  • Tissue specificity
    Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.
  • Sequence similarities
    Contains 1 EGF-like domain.
    Contains 2 F5/8 type C domains.
  • Post-translational
    Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273.
  • Cellular localization
    Membrane. Secreted.
  • Information by UniProt
  • Alternative names
    • BA46
    • Breast epithelial antigen BA46
    • Contains Medin
    • EDIL1
    • HMFG
    • hP47
    • HsT19888
    • Lactadherin
    • Lactadherin [Precursor]
    • Medin
    • MFG E8
    • MFG-E8
    • MFGE8
    • MFGM
    • Milk fat globule EGF factor 8
    • Milk fat globule EGF factor 8 protein
    • Milk fat globule-EGF factor 8
    • O acetyl disialoganglioside synthase
    • OAcGD3S
    • SED1
    • SPAG10
    • Sperm associated antigen 10
    • Sperm surface protein hP47
    see all
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab100724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse Milk Fat Globule 1 ELISA Kit images

  • Representative standard curve using ab100724


References for Mouse Milk Fat Globule 1 ELISA Kit (ab100724)

ab100724 has not yet been referenced specifically in any publications.

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