Overview

  • Product name
    Mouse Milk Fat Globule 1 ELISA Kit
    See all Milk Fat Globule 1 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 1 pg/ml
  • Range
    0.823 pg/ml - 600 pg/ml
  • Recovery

    98 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 110.1 102% - 120%
    Serum 96.1 90% - 108%
    Plasma 88.7 73% - 108%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Milk Fat Globule 1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of mouse Milk Fat Globule 1 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for mouse Milk Fat Globule 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Milk Fat Globule 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Milk Fat Globule 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Milk Fat Globule 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get better reproducibility in only 90 minutes with Mouse Milk Fat Globule 1 ELISA Kit (ab208981) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab100724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100724

Protocols

References

ab100724 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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