Mouse Milk Fat Globule 1 Multiplex Immunoassay Kit (ab221530)

Overview

  • Product name
    Mouse Milk Fat Globule 1 Multiplex Immunoassay Kit
    See all Milk Fat Globule 1 kits
  • Detection method
    Flow cytometry-fluorescent
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Serum 36 2.41%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 5.11%
  • Sample type
    Cell culture supernatant, Urine, Serum, Other biological fluids, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Sensitivity
    9.99 pg/ml
  • Range
    41.2 pg/ml - 90000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 90 87% - 95%
    Cell culture media 109 87% - 120%
    Heparin Plasma 86 75% - 93%
    EDTA Plasma 85 81% - 88%
    Citrate Plasma 90 83% - 99%

  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Quantify up to 75 proteins and peptides per well, using just 12.5 µL of sample, with FirePlex® immunoassays. The assay takes approximately 3.5 hours, followed by particle analysis using a compatible flow cytometer and data analysis using our free FirePlex Analysis Workbench software.


    Benefits of the assay


    - Quantify both low and high abundance analytes with a typical sensitivity of 0.5 pg /mL and a fully extended dynamic range spanning 4.5 logs


    - Choose from hundreds of analytes to easily build your own custom panel, use a convenient pre-mixed pre-designed panel, or add new analytes to a pre-designed panel


    - Consistent batch-to-batch performance with assays built using the same recombinant monoclonal antibody pairs as our SimpleStep ELISA® and Matched Antibody Pair Kits


    - One FirePlex particle platform for both multiplex miRNA assays and multiplex immunoassays


    Find out more about multiplex immunoassays using FirePlex particles here


    Important - Use of the FirePlex Multiplex Immunoassay Kit also requires the purchase of:


    - a) Mouse Multiplex Immunoassay Core Kit, ab209499; and


    - b) Multiplex Immunoassay Protein Standard Mix, such as ab221503.


    FirePlex® is a registered trademark in the United States and is an unregistered trademark elsewhere.

  • Notes

    The following information provides the sensitivity range and recommended starting dilutions for validated biological samples.

    Sensitivity range - 41.2 - 90,000 pg/mL

    Recommended Dilution Range
    Serum Plasma-Citrate Plasma-EDTA Plasma-Heparin Cell Culture Supernatant Urine BAL
    1:4   1:4  1:4 1:4  1:4  1:4  1:4

     

  • Platform
    Flow

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    75X Mouse Milk Fat Globule 1 Capture Particles Particle Code - 12 1 unit
    Mouse Milk Fat Globule 1 Biotin Detector {Lyophilized} 1 unit
  • Research areas
  • Function
    Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.
    Medin is the main constituent of aortic medial amyloid.
  • Tissue specificity
    Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.
  • Sequence similarities
    Contains 1 EGF-like domain.
    Contains 2 F5/8 type C domains.
  • Post-translational
    modifications
    Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273.
  • Cellular localization
    Membrane. Secreted.
  • Information by UniProt
  • Alternative names
    • BA46
    • Breast epithelial antigen BA46
    • EDIL1
    • HMFG
    • hP47
    • HsT19888
    • Lactadherin
    • Medin
    • MFG-E8
    • MFGE8
    • MFGM
    • MFGM_HUMAN
    • Milk fat globule EGF factor 8
    • Milk fat globule EGF factor 8 protein
    • Milk fat globule-EGF factor 8
    • O acetyl disialoganglioside synthase
    • OAcGD3S
    • SED1
    • SPAG10
    • Sperm associated antigen 10
    • Sperm surface protein hP47
    see all
  • Database links

Images

  • Mean background-subtracted data values are graphed (+/- SD).

  • The concentrations of Milk Fat Globule 1 were interpolated from the Milk Fat Globule 1 standard curves and corrected for sample dilution. 1X diluted samples are as follows: serum 25%, plasma (EDTA) 25%, plasma (heparin) 25%, and plasma (citrate) 25%.  The interpolated dilution factor corrected mean values are plotted.  The mean Milk Fat Globule 1 concentration was determined to be 7936 pg/mL in serum, 5612 pg/mL in plasma (EDTA), 9051 pg/mL in plasma (heparin), and 3360 pg/mL in plasma (citrate).

  • The concentrations of Milk Fat Globule 1 were interpolated from the Milk Fat Globule 1 standard curves and corrected for sample dilution. 1X diluted samples are as follows: 25% unstimulated RAW 264.7 and 25% stimulated RAW 264.7 supernatants.  The interpolated dilution factor corrected mean values are plotted.  The mean Milk Fat Globule 1 concentration was determined to be 678 pg/mL in unstimulated RAW 264.7 and 135 pg/mL in stimulated RAW 264.7 supernatants.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  The mean Milk Fat Globule 1 concentration was determined to be 11.8 ng/mL with a range of 6.60-16.2 ng/mL.

  • The concentrations of Milk Fat Globule 1 were interpolated from the Milk Fat Globule 1 standard curves and corrected for sample dilution. 1X diluted samples are as follows: 25% urine, 25% pregnant urine, 25% pregnant serum, 25% pregnant plasma (EDTA), and 25% bronchial lavage (BAL).  The interpolated dilution factor corrected values are plotted.  The mean Milk Fat Globule 1 concentration was determined to be 30.6 ng/mL in urine, 21.9 ng/mL in pregnant urine, 49.5 ng/mL in pregnant serum, 25.2 ng/mL in pregnant plasma (EDTA), and 0.582 ng/mL in bronchial lavage (BAL).

References

ab221530 has not yet been referenced specifically in any publications.

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