Mouse MIP-1 gamma ELISA Kit (CCL9) (ab155449)
Key features and details
- Sensitivity: 1 pg/ml
- Range: 1 pg/ml - 250 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse MIP-1 gamma ELISA Kit (CCL9)
See all Macrophage Inflammatory Protein 1 gamma kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 10% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 1 pg/ml -
Range
1 pg/ml - 250 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum = 83.5 75% - 91% Plasma = 90.1 81% - 101% Cell culture media = 99.02 95% - 103% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s MIP-1 (CCL9) gamma Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse MIP-1 gamma in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse MIP-1 (CCL9 gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP-1 gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP-1 gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP-1 gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-mouse MIP-1 gamma 2 vials MIP-1 gamma Microplate (12 x 8 wells) 1 unit Recombinant Mouse MIP-1 gamma Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors. -
Tissue specificity
Expressed mainly in the liver, lung, and the thymus, although some expression has been detected in a wide variety of tissues except brain. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CCF18
- Ccl9
- CCL9(31-101)
see all -
Database links
- Entrez Gene: 20308 Mouse
- SwissProt: P51670 Mouse
- Unigene: 416125 Mouse
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab155449 has been referenced in 1 publication.
- Sheng D et al. Oral S2-Ag85 DNA Vaccine Activated Intestinal Cell dsDNA and RNA Sensors to Promote the Presentation of Intestinal Antigen. J Immunol Res 2022:7200379 (2022). PubMed: 35465352