Overview

  • Product name
    Mouse MIP-1 gamma ELISA Kit (CCL9)
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 1 pg/ml
  • Range
    1 pg/ml - 250 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum = 83.5 75% - 91%
    Plasma = 90.1 81% - 101%
    Cell culture media = 99.02 95% - 103%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s MIP-1 (CCL9) gamma Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse MIP-1 gamma in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse MIP-1 (CCL9 gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP-1 gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP-1 gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP-1 gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab155449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab155449 - Assay Diluent B.

  • Representative standard curve using ab155449 -  Assay Diluent A.

Protocols

References

ab155449 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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