Mouse MIP2 ELISA Kit (CXCL2)
(ab100727)- Datasheet
- Specific References
- Protocols
Overview
-
Product name
-
Detection methodColorimetric
-
Sample typeCell culture supernatant, Plasma
-
Assay typeSandwich (quantitative)
-
Sensitivity< 8 pg/ml
-
Range4.1 pg/ml - 1000 pg/ml
-
Recovery
90 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 94.58 86% - 104% Plasma 87.94 82% - 98% -
Assay durationMultiple steps standard assay
-
Species reactivityReacts with: Mouse
-
Product overview
Abcam’s MIP2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP2 in plasma and cell culture supernatants. (Mouse MIP2 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for mouse MIP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Mouse MIP2 in normal serum with ab100727, only in serum spiked with Mouse MIP2.
-
Tested applicationsSuitable for: Sandwich ELISAmore details
-
PlatformMicroplate
Properties
-
Storage instructionsStore at -20°C. Please refer to protocols.
-
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-mouse MIP2 2 vials MIP2 Microplate (12 x 8 wells) 1 unit Recombinant Mouse MIP2 Standard 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
FunctionProduced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
-
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
-
Post-translational
modificationsThe N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells. -
Cellular localizationSecreted.
- Information by UniProt
-
Alternative names
- C-X-C motif chemokine 2
- Chemokine (C-X-C motif) ligand 2
- CINC-2a
see all -
Database links
- Entrez Gene: 20310 Mouse
- SwissProt: P10889 Mouse
Applications
Our Abpromise guarantee covers the use of ab100727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Mouse MIP2 ELISA Kit (CXCL2)
images-
Typical standard curveRepresentative standard curve using ab100727
-
Typical standard curveRepresentative standard curve using ab100727
Protocols
References for
Mouse MIP2 ELISA Kit (CXCL2)
(ab100727)ab100727 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"