Overview

  • Product nameMouse MIP2 ELISA Kit (CXCL2)
    See all CXCL2 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 8 pg/ml
  • Range
    4.1 pg/ml - 1000 pg/ml
  • Recovery

    90 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.58 86% - 104%
    Serum 88.58 83% - 99%
    Plasma 87.94 82% - 98%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s MIP2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP2 in serum, plasma and cell culture supernatants. (Mouse MIP2 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for mouse MIP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionProduced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • C-X-C motif chemokine 2
    • Chemokine (C-X-C motif) ligand 2
    • CINC-2a
    • Cxcl2
    • CXCL2_HUMAN
    • Gro beta
    • Gro-beta
    • GRO-beta(5-73)
    • GRO-beta-T
    • GRO2
    • GRO2 oncogene
    • GROb
    • Growth-regulated protein beta
    • Hematopoietic synergistic factor
    • HSF
    • Macrophage inflammatory protein 2-alpha
    • Melanoma growth stimulatory activity beta
    • MGSA beta
    • MGSA-b
    • MIP 2
    • MIP 2a
    • MIP2
    • MIP2-alpha
    • MIP2A
    • SB-251353
    • SCYB2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse MIP2 ELISA Kit (CXCL2) images

  • Representative standard curve using ab100727

  • Representative standard curve using ab100727

Protocols

References for Mouse MIP2 ELISA Kit (CXCL2) (ab100727)

ab100727 has not yet been referenced specifically in any publications.

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