|Sample type||Average %||Range|
|Cell culture supernatant||85.6||78% - 101%|
|Serum||107.7||82% - 132%|
|Plasma||119.5||83% - 140%|
Abcam’s MIP3 beta Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP-3- beta in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse MIP3 beta coated on a 96- well plate. Standards and samples are pipetted into the wells and MIP3 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP3 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|260X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent||1 x 15ml|
|Biotinylated anti-mouse MIP3 beta||2 vials|
|MIP3 beta Microplate (12 x 8 wells)||1 unit|
|Recombinant Mouse MIP3 beta Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100729 has not yet been referenced specifically in any publications.