Overview

  • Product nameMouse MMP2 ELISA Kit
    See all MMP2 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 25 pg/ml
  • Range
    27.43 pg/ml - 20000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.21 86% - 104%
    Serum 107.6 96% - 127%
    Plasma 105.6 95% - 120%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s MMP2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MMP2 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse MMP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MMP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionUbiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
    -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
    PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
  • Tissue specificityProduced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
  • Involvement in diseaseDefects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
    The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
  • Cellular localizationSecreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
  • Information by UniProt
  • Alternative names
    • 72 kDa gelatinase
    • 72 kDa type IV collagenase
    • CLG 4
    • CLG 4A
    • CLG4
    • CLG4A
    • Collagenase type IV A
    • EC 3.4.24.24
    • Gelatinase A
    • Gelatinase alpha
    • Gelatinase neutrophil
    • Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
    • Matrix metalloproteinase 2
    • Matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase)
    • Matrix metalloproteinase II
    • Matrix metalloproteinase-2
    • MMP 2
    • MMP II
    • MMP-2
    • MMP2
    • MMP2_HUMAN
    • MONA
    • Neutrophil gelatinase
    • PEX
    • TBE 1
    • TBE-1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse MMP2 ELISA Kit images

  • MMP2 measured in biological fluids showing quantity (pg) per mL tested sample

  • Representative standard curve using ab100730

Protocols

References for Mouse MMP2 ELISA Kit (ab100730)

This product has been referenced in:
  • Yu S  et al. Global ablation of the mouse Rab11a gene impairs early embryogenesis and matrix metalloproteinase secretion. J Biol Chem 289:32030-43 (2014). Read more (PubMed: 25271168) »

See 1 Publication for this product

Product Wall

The kit will definitely detect the pro form (as the pro form was the antibody immunogen) but it is not known whether the kit will also detect the active form as epitope mapping has not been performed. Detection of the active form would be expected thou...

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Thank you for contacting us.

I now have further information to share with you in regards to the MMP2 ELISA kits, ab100606 and ab100730. The antibodies used within these kits have used the following immunogens:

ab100606 capture antib...

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Thank you for contacting us.  This MMP2 Mouse ELISA Kit recognizes both the pro and mature form (total).  I hope this helps, please let me know if you need any additional information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"