Mouse MMP3 ELISA Kit (ab100731)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 13.72 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse MMP3 ELISA Kit
See all MMP3 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
13.72 pg/ml - 10000 pg/ml -
Recovery
100 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 117.4 106% - 130% Serum 120.6 101% - 140% Plasma 111.6 101% - 137% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s MMP3 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MMP3 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse MMP3 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MMP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated anti-Mouse MMP-3 2 vials MMP-3 Microplate (12 x 8 wells) 1 unit Recombinant Mouse MMP-3 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- CHDS6
- Matrix metalloproteinase 3
- Matrix metalloproteinase 3 preproprotein
see all -
Database links
- Entrez Gene: 17392 Mouse
- SwissProt: P28862 Mouse
- Unigene: 4993 Mouse
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab100731 has been referenced in 2 publications.
- Brelstaff JH et al. Microglia become hypofunctional and release metalloproteases and tau seeds when phagocytosing live neurons with P301S tau aggregates. Sci Adv 7:eabg4980 (2021). PubMed: 34669475
- Yamakawa M et al. Porphyromonas gingivalis infection exacerbates the onset of rheumatoid arthritis in SKG mice. Clin Exp Immunol 186:177-189 (2016). PubMed: 27465496