The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use 1µg for 106 cells. ab106163-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Mouse monoclonal 2A 8F4 Anti-Rat IgG2a heavy chain (FITC) images
Flow Cytometry - Mouse monoclonal [2A 8F4] Secondary Antibody to Rat IgG2a - heavy chain (FITC) (ab99665)
Top: (Negative control) ab99665 being used against BALB/c spleenocytes at 1 µg/106 cells.
Bottom: ab99665 staining BALB/c spleenocytes incubated with rat anti-mouse CD8 (IgG2a isotype) at 1 µg/106 cells.
References for Mouse monoclonal 2A 8F4 Anti-Rat IgG2a heavy chain (FITC) (ab99665)
has not yet been referenced specifically in any publications.
Publishing research using ab99665? Please let us know so that we can cite the reference in this datasheet.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Get resources and offers direct to your inboxSign up